Conducted by Naturallythinking Labs, The Tramsheds, Coomber Way, Croydon, Surrey, CR0 4TQ
Report Date: 06/01/2017 | Reference: ZCUSTOMER100196663B
The Test for Efficacy of Antimicrobial Preservation demonstrates the effectiveness of a substance when used as a preservative within a cosmetic formula to stop the growth of such pathogenic organisms as Aspergillus brasiliensis, Candida albicans, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus.
Customer: Katjusa Reja Mozetic
Sample Description: Aloe Vera
Test Performed: Preservative Efficacy Test - Efficacy of Antimicrobal Preservation (BS EN ISO 11930:2012)
Reference: ISO11930
Sample preparation:
Aspergillus brasiliensis, Candida albicans, E-Coli, Pseudomonas aeruginosa, and Staphylococcus aureus are used to challenge the specimen for twenty-eight (28) days. Microorganism survival is monitored after seven (7), fourteen (14) and twenty-eight (28) days.
Micro-organism
ATCC Number
A. brasiliensis
16404
C. albicans
10231
E-Coli
8739
P. aeruginosa
9027
S. aureus
6538
Colony Forming Units per gram
Bacteria
Moulds
Yeasts
Initial TVC
<10
< 10
< 10
Colony Forming Units /g
Time
P. aeruginosa
S. aureus
A. brasiliensis
C. albicans
E-Coli
0 control
2.3 x 106
3.8 x 106
1.8 x 105
1.6 x 106
3.1 x 106
7 days
< 10
< 10
-
< 10
< 10
14 days
< 10
< 10
< 10
< 10
< 10
28 days
< 10
< 10
INCREASE
< 10
< 10
The results apply only to the sample(s) tested. When assessed against the ISO11930:2012 (Cosmetics - Microbiology - Evaluation of the antimicrobal protection of a cosmetic products) criteria A, This sample Fails.