NATURALLYTHINKING LABORATORY TESTS

Test for Efficacy of Antimicrobial Preservation

Conducted by Naturallythinking Labs, The Tramsheds, Coomber Way, Croydon, Surrey, CR0 4TQ
Report Date: 06/01/2017 | Reference: ZCUSTOMER100196663B

What the test means

The Test for Efficacy of Antimicrobial Preservation demonstrates the effectiveness of a substance when used as a preservative within a cosmetic formula to stop the growth of such pathogenic organisms as Aspergillus brasiliensis, Candida albicans, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus.

Customer: Katjusa Reja Mozetic

Sample Description: Aloe Vera

Test Performed: Preservative Efficacy Test - Efficacy of Antimicrobal Preservation (BS EN ISO 11930:2012)

Reference: ISO11930

Sample preparation:

Aspergillus brasiliensis, Candida albicans, E-Coli, Pseudomonas aeruginosa, and Staphylococcus aureus are used to challenge the specimen for twenty-eight (28) days. Microorganism survival is monitored after seven (7), fourteen (14) and twenty-eight (28) days.

Certificate of Performance:

Micro-organism

ATCC Number

A. brasiliensis

16404

C. albicans

10231

E-Coli

8739

P. aeruginosa

9027

S. aureus

6538

 

Colony Forming Units per gram

 

Bacteria

Moulds

Yeasts

 Initial TVC

<10

< 10

< 10

Colony Forming Units /g

Time

P. aeruginosa

S. aureus

A. brasiliensis

C. albicans

E-Coli

0 control

2.3 x 10⁶

3.8 x 10⁶

1.8 x 10⁵

1.6 x 10⁶

3.1 x 10⁶

7 days

< 10

< 10

-

< 10

< 10

14 days

< 10

< 10

< 10

< 10

< 10

28 days

< 10

< 10

INCREASE

< 10

< 10

---

Conclusion ( INADEQUATE / FAIL):

The results apply only to the sample(s) tested. When assessed against the ISO11930:2012 (Cosmetics - Microbiology - Evaluation of the antimicrobal protection of a cosmetic products) criteria A, This sample Fails.

Paige Chandler
BSc BioChemisty (Imperial College London)
The Tramsheds, Coomber Way, Croydon, Surrey, CR0 4TQ
Tel: 020 8689 6489
Web: www.naturallythinking.com